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University Paris Diderot, CNRS UMR 3571, Institut Pasteur

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

Three-dimensional Quantification of Dendritic Spines from Pyramidal Neurons Derived from Human Induced Pluripotent Stem Cells

Laura Gouder^1,2,3, Jean-Yves Tinevez⁴, Hany Goubran-Botros^1,2,3, Alexandra Benchoua⁵, Thomas Bourgeron^1,2,3, Isabelle Cloëz-Tayarani^1,2,3

¹Human Genetics and Cognitive Functions, Institut Pasteur, ²CNRS URA 2182 'Genes, synapses and cognition', Institut Pasteur, ³Human Genetics and Cognitive Functions, Université Paris Diderot, Sorbonne Paris Cité, ⁴Plateforme d' Imagerie Dynamique, Imagopole, CiTech, Institut Pasteur, ⁵Neuroplasticity and Therapeutics, CECS, I-STEM, AFM, Evry

Dendritic spines of pyramidal neurons are the sites of most excitatory synapses in mammalian brain cortex. This method describes a 3D quantitative analysis of spine morphologies in human cortical pyramidal glutamatergic neurons derived from induced pluripotent stem cells.

Behavior

Recording Mouse Ultrasonic Vocalizations to Evaluate Social Communication

Allain-Thibeault Ferhat¹, Nicolas Torquet², Anne-Marie Le Sourd¹, Fabrice de Chaumont³, Jean-Christophe Olivo-Marin³, Philippe Faure², Thomas Bourgeron¹, Elodie Ey¹

¹Human Genetics and Cognitive Functions, University Paris Diderot, CNRS UMR 3571, Institut Pasteur, ²Neurophysiology and Behavior, University Pierre et Marie Curie Paris 6, CNRS UMR 7102, ³Bio Image Analysis, CNRS URA 2582, Institut Pasteur

Mouse ultrasonic vocalizations are used as proxies to model the genetic bases of vocal communication deficits in mouse models for neuropsychiatric disorders. The present protocol describes three experimental contexts that reliably elicit ultrasonic vocalizations from pups (throughout development) and adult mice (same-sex interactions, male-estrus female interactions).