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Narayana Nethralaya Foundation, Narayana Health City

2 ARTICLES PUBLISHED IN JoVE

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Developmental Biology

An Enzyme- and Serum-free Neural Stem Cell Culture Model for EMT Investigation Suited for Drug Discovery
Martin H. M. Sailer 1, Durga Sarvepalli 2, Catherine Brégère 3, Urs Fisch 3, Marin Guentchev 4, Michael Weller 6, Raphael Guzman 3, Bernhard Bettler 1, Arkasubhra Ghosh *2, Gregor Hutter *5
1Dept. of Biomedicine, Pharmacenter, University of Basel, 2Molecular Signalling and Gene Therapy, Narayana Nethralaya Foundation, Narayana Health City, 3Brain Ischemia and Regeneration, Department of Biomedicine, University Hospital Basel, 4Department of Neurosurgery, Klinikum Idar-Oberstein, 5Department of Neurosurgery and Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, 6Department of Neurology, Laboratory of Molecular Neuro Oncology, University Hospital of Zurich

Epithelial to mesenchymal transition (EMT) allows cancers to become invasive. To investigate EMT, a neural stem cell (NSC)-based in vitro model devoid of serum and enzymes is described. This standardized system allows quantitative and qualitative assessment of cell migration, gene and protein expression. The model is suited for drug discovery.

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Immunology and Infection

Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles
Praveen Kumar Balne 1,2, Veonice Bijin AU 3, Louis Tong 2, Arkasubhra Ghosh 4, Mukesh Agrawal 5, John Connolly 3, Rupesh Agrawal 1
1National Healthcare Group Eye Institute, Tan Tock Seng Hospital, 2Singapore Eye Research Institute (SERI), 3Institute of Molecular and Cell Biology, A*STAR, 4GROW Research Laboratory, Narayana Nethralaya Foundation, 5Vimta Labs Limited

Analysis of tear film cytokines helps in studying various ocular diseases. Bead based multiplex assays are simple and sensitive and enable the testing of multiple targets in samples with small volumes. Here we describe a protocol for tear film cytokine profiling a using bead based multiplex assay.

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