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Narayana Nethralaya Foundation, Narayana Health City

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

An Enzyme- and Serum-free Neural Stem Cell Culture Model for EMT Investigation Suited for Drug Discovery

Martin H. M. Sailer¹, Durga Sarvepalli², Catherine Brégère³, Urs Fisch³, Marin Guentchev⁴, Michael Weller⁶, Raphael Guzman³, Bernhard Bettler¹, Arkasubhra Ghosh^*2, Gregor Hutter^*5

¹Dept. of Biomedicine, Pharmacenter, University of Basel, ²Molecular Signalling and Gene Therapy, Narayana Nethralaya Foundation, Narayana Health City, ³Brain Ischemia and Regeneration, Department of Biomedicine, University Hospital Basel, ⁴Department of Neurosurgery, Klinikum Idar-Oberstein, ⁵Department of Neurosurgery and Institute for Stem Cell Biology and Regenerative Medicine, Stanford University, ⁶Department of Neurology, Laboratory of Molecular Neuro Oncology, University Hospital of Zurich

Epithelial to mesenchymal transition (EMT) allows cancers to become invasive. To investigate EMT, a neural stem cell (NSC)-based in vitro model devoid of serum and enzymes is described. This standardized system allows quantitative and qualitative assessment of cell migration, gene and protein expression. The model is suited for drug discovery.

Immunology and Infection

Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles

Praveen Kumar Balne^1,2, Veonice Bijin AU³, Louis Tong², Arkasubhra Ghosh⁴, Mukesh Agrawal⁵, John Connolly³, Rupesh Agrawal¹

¹National Healthcare Group Eye Institute, Tan Tock Seng Hospital, ²Singapore Eye Research Institute (SERI), ³Institute of Molecular and Cell Biology, A*STAR, ⁴GROW Research Laboratory, Narayana Nethralaya Foundation, ⁵Vimta Labs Limited

Analysis of tear film cytokines helps in studying various ocular diseases. Bead based multiplex assays are simple and sensitive and enable the testing of multiple targets in samples with small volumes. Here we describe a protocol for tear film cytokine profiling a using bead based multiplex assay.