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The Blood Center of Wisconsin

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

High-throughput Detection Method for Influenza Virus

Pawan Kumar¹, Allison E. Bartoszek¹, Thomas M. Moran², Jack Gorski³, Sanjib Bhattacharyya⁴, Jose F. Navidad⁴, Monica S. Thakar^1,5, Subramaniam Malarkannan^1,6

¹Laboratory of Molecular Immunology and Immunotherapy, Blood Research Institute, ²Department of Microbiology, Mount Sinai School of Medicine , ³Laboratory of Molecular Genetics, Blood Research Institute, ⁴City of Milwaukee Health Department Laboratory, ⁵Division of Hematology-Oncology/BMT, Children's Hospital of Wisconsin, Medical College of Wisconsin , ⁶Division of Hematology and Oncology, Dept Medicine, Medical College of Wisconsin

This method describes the use of Infrared dye based imaging system for detection of H1N1 in bronchioalveolar lavage (BAL) fluid of infected mice at a high sensitivity. This methodology can be performed in a 96- or 384-well plate, requires <10 μl volume of test material and has the potential for concurrent screening of multiple pathogens.

Immunology and Infection

Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells

Arash Nanbakhsh¹, Brad Best², Matthew Riese³, Sridhar Rao⁴, Li Wang⁵, Jeffrey Medin⁶, Monica S. Thakar⁶, Subramaniam Malarkannan^1,5,6,7

¹Laboratory of Molecular Immunology and Immunotherapy, Blood Research Institute, The Blood Center of Wisconsin, ²Vector Core Lab, Blood Research Institute, The Blood Center of Wisconsin, ³Laboratory of Lymphocyte Biology, Blood Research Institute, The Blood Center of Wisconsin, ⁴Laboratory of Stem Cell Transcriptional Regulation, Blood Research Institute, The Blood Center of Wisconsin, ⁵Department of Microbiology and Immunology, The Medical College of Wisconsin, ⁶Department of Pediatrics, The Medical College of Wisconsin, ⁷Department of Medicine, The Medical College of Wisconsin

The goal of this study was to formulate technologies that allow for successful gene transduction in primary natural killer (NK) cells. The dextran-mediated lentiviral transduction of human or mouse primary NK cells results in higher gene expression efficiencies. This method of gene transduction will vastly improve NK cell genetic manipulation.