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A.N. Tupolev Kazan National Research Technical University

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Loading a Calcium Dye into Frog Nerve Endings Through the Nerve Stump: Calcium Transient Registration in the Frog Neuromuscular Junction

Dmitry V. Samigullin^1,2,3, Eduard F. Khaziev^1,2,3, Nikita V. Zhilyakov^1,2, Ellya A. Bukharaeva^1,2, Eugeny E. Nikolsky^1,2,4

¹Laboratory of Biophysics of Synaptic Processes, Kazan Scientific Centre, Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences, ²Open Laboratory of Neuropharmacology, Kazan Federal University, ³Department of Radiophotonics and Microwave Technologies, A.N. Tupolev Kazan National Research Technical University, ⁴Department of Medical and Biological Physics, Kazan State Medical University

Here, we describe a method for loading a calcium-sensitive dye through the frog nerve stump into the nerve endings. We also present a protocol for the recording and analysis of fast calcium transients in the peripheral nerve endings.

JoVE Core

Registration of Calcium Transients in Mouse Neuromuscular Junction with High Temporal Resolution using Confocal Microscopy

Nikita V. Zhilyakov¹, Arsenii Y. Arkhipov¹, Eduard F. Khaziev^1,2, Marat A. Mukhamedyarov³, Dmitry V. Samigullin^1,2

¹Laboratory of Biophysics of Synaptic Processes, Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center of RAS, ²Department of Radiophotonics and Microwave Technologies, Kazan National Research Technical University named after A.N. Tupolev, ³Department of Normal Physiology, Kazan State Medical University

The protocol describes the method of loading a fluorescent calcium dye through the cut nerve into mouse motor nerve terminals. In addition, a unique method for recording fast calcium transients in the peripheral nerve endings using confocal microscopy is presented.