Results: Mouse Nerve Terminals Loaded with the Ca2+ Indicator and Representative Trace of Calcium Signal
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Conclusion
Transkript
Estimation of a presynaptic calcium level is a key task in studying synaptic transmission. Studying calcium signaling is also important for finding ways to treat neurodegenerative diseases. The main advantage of the presented loading technique is
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The protocol describes the method of loading a fluorescent calcium dye through the cut nerve into mouse motor nerve terminals. In addition, a unique method for recording fast calcium transients in the peripheral nerve endings using confocal microscopy is presented.