Chiesi Farmaceutici S.p.A.
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
An IL-8 Transiently Transgenized Mouse Model for the In Vivo Long-term Monitoring of Inflammatory Responses
Gabriella Bergamini *1, Fabio Stellari *2, Angela Sandri *3, Maria M. Lleo 3, Gaetano Donofrio 4, Francesca Ruscitti 5,2, Federico Boschi 6, Andrea Sbarbati 7, Gino Villetti 2, Paola Melotti 8, Claudio Sorio 1
1Department of Medicine, General Pathology Division, Cystic Fibrosis Translational Research Laboratory "D. Lissandrini", University of Verona, 2Corporate Preclinical R&D, Chiesi Farmaceutici S.p.A., 3Department of Diagnostic and Public Health, Microbiology Division, University of Verona, 4Dipartimento di Scienze Medico-Veterinarie, University of Parma, 5Department of Biomedical Biotechnological and Translational Sciences, University of Parma, 6Department of Computer Science, University of Verona, 7Department of Neurological, Biomedical and Movement Sciences, University of Verona, 8Cystic Fibrosis Regional Center (CFC), AOUI Verona
The method described here allows for the visualization of IL-8 promoter-dependent inflammation activation in the lungs of mice through non-invasive bioluminescence imaging (BLI). The same animal can be subjected to BLI multiple times for up to two months from the time of delivery of the luciferase reporter construct.
Immunology and Infection
A Multimodal Imaging Approach Based on Micro-CT and Fluorescence Molecular Tomography for Longitudinal Assessment of Bleomycin-Induced Lung Fibrosis in Mice
Francesca Ruscitti 1, Francesca Ravanetti 2, Gaetano Donofrio 2, Yanto Ridwan 3, Paula van Heijningen 3, Jeroen Essers 4, Gino Villetti 1, Antonio Cacchioli 2, Wim Vos 5, Franco Fabio Stellari 1
1Corporate Preclinical R&D, Chiesi Farmaceutici S.p.A., 2Department of Veterinary Science, University of Parma, 3Department of Molecular Genetics, Erasmus MC, 4Department of Molecular Genetics, Vascular Surgery, Radiation Oncology, Erasmus MC, 5Fluidda NV
We describe a non-invasive multimodal imaging approach based on Micro-CT and fluorescence molecular tomography for longitudinal assessment of the mouse lung fibrosis model induced by double intratracheal instillation of bleomycin.
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