Chiesi Farmaceutici S.p.A.

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

An IL-8 Transiently Transgenized Mouse Model for the In Vivo Long-term Monitoring of Inflammatory Responses

Gabriella Bergamini^*1, Fabio Stellari^*2, Angela Sandri^*3, Maria M. Lleo³, Gaetano Donofrio⁴, Francesca Ruscitti^5,2, Federico Boschi⁶, Andrea Sbarbati⁷, Gino Villetti², Paola Melotti⁸, Claudio Sorio¹

¹Department of Medicine, General Pathology Division, Cystic Fibrosis Translational Research Laboratory "D. Lissandrini", University of Verona, ²Corporate Preclinical R&D, Chiesi Farmaceutici S.p.A., ³Department of Diagnostic and Public Health, Microbiology Division, University of Verona, ⁴Dipartimento di Scienze Medico-Veterinarie, University of Parma, ⁵Department of Biomedical Biotechnological and Translational Sciences, University of Parma, ⁶Department of Computer Science, University of Verona, ⁷Department of Neurological, Biomedical and Movement Sciences, University of Verona, ⁸Cystic Fibrosis Regional Center (CFC), AOUI Verona

The method described here allows for the visualization of IL-8 promoter-dependent inflammation activation in the lungs of mice through non-invasive bioluminescence imaging (BLI). The same animal can be subjected to BLI multiple times for up to two months from the time of delivery of the luciferase reporter construct.

Immunology and Infection

A Multimodal Imaging Approach Based on Micro-CT and Fluorescence Molecular Tomography for Longitudinal Assessment of Bleomycin-Induced Lung Fibrosis in Mice

Francesca Ruscitti¹, Francesca Ravanetti², Gaetano Donofrio², Yanto Ridwan³, Paula van Heijningen³, Jeroen Essers⁴, Gino Villetti¹, Antonio Cacchioli², Wim Vos⁵, Franco Fabio Stellari¹

¹Corporate Preclinical R&D, Chiesi Farmaceutici S.p.A., ²Department of Veterinary Science, University of Parma, ³Department of Molecular Genetics, Erasmus MC, ⁴Department of Molecular Genetics, Vascular Surgery, Radiation Oncology, Erasmus MC, ⁵Fluidda NV

We describe a non-invasive multimodal imaging approach based on Micro-CT and fluorescence molecular tomography for longitudinal assessment of the mouse lung fibrosis model induced by double intratracheal instillation of bleomycin.