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University of Nevada

5 ARTICLES PUBLISHED IN JoVE

Behavior

Task Interruption and Resumption Paradigm for Testing the Activation and Pursuit of an Abstract Thinking Goal

Jae-Eun Namkoong¹, Marlone D. Henderson²

¹Managerial Sciences Department, University of Nevada, ²Department of Psychology, University of Texas

This protocol was designed to test the activation and pursuit of cognitive goals (e.g., an abstract thinking goal) using the task interruption and resumption paradigm. The protocol is suitable for cognitive goals that are automatically pursued once activated, as the distraction procedure prevents goal pursuit during the interruption period.

JoVE Journal

Tracking Drosophila Larval Behavior in Response to Optogenetic Stimulation of Olfactory Neurons

David A. Clark^1,2, Donovan Kohler¹, America Mathis¹, Eryn Slankster¹, Samipya Kafle¹, Seth R. Odell^1,2, Dennis Mathew^1,2

¹Department of Biology, MS-0314, University of Nevada, ²Integrated Neuroscience Graduate Program, University of Nevada

This protocol analyzes navigational behavior of Drosophila larva in response to simultaneous optogenetic stimulation of its olfactory neurons. Light of 630 nm wavelength is used to activate individual olfactory neurons expressing a red-shifted channel rhodopsin. Larval movement is simultaneously tracked, digitally recorded, and analyzed using custom-written software.

Neuroscience

Ex Vivo Imaging of Cell-specific Calcium Signaling at the Tripartite Synapse of the Mouse Diaphragm

Dante J. Heredia¹, Grant W. Hennig², Thomas W. Gould¹

¹Department of Physiology and Cell Biology, School of Medicine, University of Nevada, ²Department of Pharmacology, Larner College of Medicine, University of Vermont

Here we present a protocol to image calcium signaling in populations of individual cell types at the murine neuromuscular junction.

Biochemistry

Recombinant Production of Bifidobacterial Endoglycosidases for N-glycan Release

Berfin Sucu¹, Ayşe Bayraktar^1,2, Hatice Duman¹, Ayşenur Arslan¹, Merve Kaplan¹, Melda Karyelioğlu¹, Eda Ntelitze¹, Taner Taştekin¹, Seray Yetkin¹, Melih Ertürk², Steven A. Frese^3,4, Bethany M. Henrick^4,5, Hacı Mehmet Kayili⁶, Bekir Salih⁷, Sercan Karav¹

¹Department of Molecular Biology and Genetics, Çanakkale Onsekiz Mart University, ²Uluova Dairy Farm, ³Department of Nutrition, University of Nevada, ⁴Department of Food Science and Technology, University of Nebraska, ⁵Evolve BioSystems Inc., ⁶Department of Biomedical Engineering, Karabuk University, ⁷Department of Chemistry, Hacettepe University

Bifidobacteria possess a unique genomic capability for N-glycan cleavage. Recombinantly producing these enzymes would be a promising novel tool to release bioactive N-glycans from glycoprotein-rich substrates such as colostrum.

Biochemistry

Formulation and Characterization of Bioactive Agent Containing Nanodisks

Kyle Lethcoe¹, Colin A. Fox¹, Isabel Moh¹, Matthew Swackhamer¹, Michael Karo¹, Ryan Lockhart¹, Robert O. Ryan¹

¹Department of Biochemistry and Molecular Biology, University of Nevada

Here, we describe the production and characterization of bioactive agents containing nanodisks. Amphotericin B nanodisks are taken as an example to describe the protocol in a stepwise manner.