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Boehringer Ingelheim Pharmaceuticals, Inc.

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms

Danlin Yang¹, Ajit Singh², Helen Wu¹, Rachel Kroe-Barrett¹

¹Department of Biotherapeutics Discovery, Immune Modulation and Biotherapeutics Discovery, Boehringer Ingelheim Pharmaceuticals, Inc., ²The Fu Foundation School of Engineering and Applied Science, Columbia University

We describe here protocols for the measurement of antibody-antigen binding affinity and kinetics using four commonly used biosensor platforms.

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