Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor PlatformsDanlin Yang 1, Ajit Singh 2, Helen Wu 1, Rachel Kroe-Barrett 1
1Department of Biotherapeutics Discovery, Immune Modulation and Biotherapeutics Discovery, Boehringer Ingelheim Pharmaceuticals, Inc., 2The Fu Foundation School of Engineering and Applied Science, Columbia University
We describe here protocols for the measurement of antibody-antigen binding affinity and kinetics using four commonly used biosensor platforms.
