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National Institute of Information and Communications Technology

5 ARTICLES PUBLISHED IN JoVE

Neuroscience

Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes

Naoki Ihara¹, Yuji Ikegaya^1,2, Haruki Takeuchi^1,3

¹Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, ²Center for Information and Neural Networks, National Institute of Information and Communications Technology, ³PRESTO, Japan Science and Technology Agency (JST)

Olfactory sensory neurons express a wide variety of axon-sorting molecules to establish proper neural circuitry. This protocol describes an immunohistochemical staining method to visualize combinatorial expressions of axon-sorting molecules at the axon termini of olfactory sensory neurons.

Neuroscience

Simultaneous Recordings of Cortical Local Field Potentials, Electrocardiogram, Electromyogram, and Breathing Rhythm from a Freely Moving Rat

Yu Shikano¹, Takuya Sasaki¹, Yuji Ikegaya^1,2

¹Graduate School of Pharmaceutical Sciences, The University of Tokyo, ²Center for Information and Neural Networks

This study introduces a method for the simultaneous recording of local field potentials in the brain, electrocardiograms, electromyograms, and breathing signals of a freely moving rat. This technique, which reduces experimental costs and simplifies data analysis, will contribute to the understanding of the interactions between the brain and peripheral organs.

Biology

High-Accuracy Correction of 3D Chromatic Shifts in the Age of Super-Resolution Biological Imaging Using Chromagnon

Atsushi Matsuda^1,2, Takako Koujin¹, Lothar Schermelleh³, Tokuko Haraguchi^1,2, Yasushi Hiraoka^1,2

¹Advanced ICT Research Institute Kobe, National Institute of Information and Communications Technology, ²Graduate School of Frontier Biosciences, Osaka University, ³Micron Advanced Bioimaging Unit, Department of Biochemistry, University of Oxford

Correction of chromatic shifts in three-dimensional (3D) multicolor fluorescence microscopy images is crucial for quantitative data analyses. This protocol is developed to measure and correct chromatic shifts in biological samples through acquisition of suitable reference images and processing with the open-source software, Chromagnon.

Behavior

Tactile Semiautomatic Passive-Finger Angle Stimulator (TSPAS)

Wu Wang¹, Jiajia Yang^2,4, Yinghua Yu^2,3,4, Qiong Wu^5,2, Satoshi Takahashi², Yoshimichi Ejima², Jinglong Wu^6,2

¹Cognitive Neuroscience Laboratory, Graduate School of Natural Science and Technology, Okayama University, ²Cognitive Neuroscience Laboratory, Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, ³Center for Information and Neural Networks, National Institute of Information and Communications Technology, ⁴Section on Functional Imaging Methods, National Institute of Mental Health, ⁵School of Education, Suzhou University of Science and Technology, ⁶Beijing Institute of Technology

Presented is the tactile semiautomated passive-finger angle stimulator TSPAS, a new way to assess tactile spatial acuity and tactile angle discrimination using a computer-controlled tactile stimulus system that applies raised angle stimuli to a subject's passive fingerpad, while controlling for movement speed, distance, and contact duration.

Neuroscience

Reliable Acquisition of Electroencephalography Data during Simultaneous Electroencephalography and Functional MRI

Hui Ming Khoo¹, Yuya Fujita¹, Naoki Tani¹, Tetsuya Shimokawa², Natalja Zazubovits³, Satoru Oshino¹, Jean Gotman³, Haruhiko Kishima¹

¹Department of Neurosurgery, Osaka University Graduate School of Medicine, ²Center for Information and Neural Networks, National Institute of Information and Communications Technology, ³Montreal Neurological Institute and Hospital, McGill University

This article provides a straightforward protocol for acquiring good quality electroencephalography (EEG) data during simultaneous EEG and functional magnetic resonance imaging by utilizing readily available medical products.