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Rowan School of Osteopathic Medicine

1 ARTICLES PUBLISHED IN JoVE

Genetics

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response

Stephen D. Willis¹, A. K. M. Nawshad Hossian², Nathan Evans², Mark J. Hickman²

¹Department of Molecular Biology, Rowan School of Osteopathic Medicine, ²Department of Biological Sciences, Rowan University

Here, we present a protocol using RNA-seq to monitor mRNA levels over time during the hypoxic response of S. cerevisiae cells. This method can be adapted to analyze gene expression during any cellular response.