Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic ResponseStephen D. Willis 1, A. K. M. Nawshad Hossian 2, Nathan Evans 2, Mark J. Hickman 2
1Department of Molecular Biology, Rowan School of Osteopathic Medicine, 2Department of Biological Sciences, Rowan University
Here, we present a protocol using RNA-seq to monitor mRNA levels over time during the hypoxic response of S. cerevisiae cells. This method can be adapted to analyze gene expression during any cellular response.
