RenOVAte Biosciences Inc
2 ARTICLES PUBLISHED IN JoVE
Biology
Utero-tubal Embryo Transfer and Vasectomy in the Mouse Model
Pablo Bermejo-Alvarez 1,2, Ki-Eun Park 1,2, Bhanu P. Telugu 1,2
1Animal Bioscience and Biotechnology Laboratory, United States Department of Agriculture, 2Department of Animal and Avian Sciences, University of Maryland
Utero-tubal embryo transfer uses the utero-tubal junction as a barrier to prevent the embryo outflow that may occur when performing uterine transfer. Vasectomized males are required to obtain pseudopregnant recipients for embryo transfer. Both techniques are discussed.
Bioengineering
Efficient Generation and Editing of Feeder-free IPSCs from Human Pancreatic Cells Using the CRISPR-Cas9 System
Anjali Nandal 1,2, Barbara Mallon 3, Bhanu P. Telugu 1,2,4
1Department of Animal and Avian Sciences, University of Maryland, 2Animal Bioscience and Biotechnology Laboratory, ARS, USDA, 3NIH Stem Cell Unit, Bethesda, National Institutes of Health, 4RenOVAte Biosciences Inc
This protocol describes in detail the generation of footprint-free induced pluripotent stem cells (iPSCs) from human pancreatic cells in feeder-free conditions, followed by editing using CRISPR/Cas9 ribonucleoproteins and characterization of the modified single-cell clones.
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