University of Brasília
2 ARTICLES PUBLISHED IN JoVE
Biology
Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds
Calliandra Maria de-Souza-Silva 1, Fernanda Guilhelmelli 1, Daniel Zamith-Miranda 2,3, Marco Antônio de Oliveira 1, Joshua Daniel Nosanchuk 2,3, Ildinete Silva-Pereira *1, Patrícia Albuquerque *1,4
1Laboratory of Molecular Biology, Department of Cellular Biology, Institute of Biological Sciences, University of Brasília, 2Department of Microbiology and Immunology, Albert Einstein College of Medicine, 3Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine, 4Faculty of Ceilândia, University of Brasília
An easy and adaptable broth microdilution method for screening antifungal compounds and extracts.
Immunology and Infection
Pseudotyped Viruses As a Molecular Tool to Monitor Humoral Immune Responses Against SARS-CoV-2 Via Neutralization Assay
Tobia Fantoni *1, Michele Bissoli *1, Chiara Stefani 1, Mauro Voi 1, Alexandrina Dabija 1, Rebecca Casula 1, Domenico Luca Minafra 1, Julys da Fonseca Palmeira 2, Enrique Roberto Argañaraz 2, Martin Mayora-Neto 3, Nigel J. Temperton 3, Donato Zipeto 1, Alessandra Ruggiero 1
1Department of Neuroscience, Biomedicine and Movement, University of Verona, 2Laboratory of Molecular Neurovirology, Faculty of Health Science, University of Brasília, 3Viral Pseudotype Unit, Medway School of Pharmacy, Universities of Kent and Greenwich at Medway
Pseudotyped viruses (PVs) are replication-defective virions that are used to study host-virus interactions under safer conditions than handling authentic viruses. Presented here is a detailed protocol that shows how SARS-CoV-2 PVs can be used to test the neutralizing ability of patients' serum after COVID-19 vaccination.
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