University of Brasília

2 ARTICLES PUBLISHED IN JoVE

Biology

Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds

Calliandra Maria de-Souza-Silva¹, Fernanda Guilhelmelli¹, Daniel Zamith-Miranda^2,3, Marco Antônio de Oliveira¹, Joshua Daniel Nosanchuk^2,3, Ildinete Silva-Pereira^*1, Patrícia Albuquerque^*1,4

¹Laboratory of Molecular Biology, Department of Cellular Biology, Institute of Biological Sciences, University of Brasília, ²Department of Microbiology and Immunology, Albert Einstein College of Medicine, ³Division of Infectious Diseases, Department of Medicine, Albert Einstein College of Medicine, ⁴Faculty of Ceilândia, University of Brasília

An easy and adaptable broth microdilution method for screening antifungal compounds and extracts.

Immunology and Infection

Pseudotyped Viruses As a Molecular Tool to Monitor Humoral Immune Responses Against SARS-CoV-2 Via Neutralization Assay

Tobia Fantoni^*1, Michele Bissoli^*1, Chiara Stefani¹, Mauro Voi¹, Alexandrina Dabija¹, Rebecca Casula¹, Domenico Luca Minafra¹, Julys da Fonseca Palmeira², Enrique Roberto Argañaraz², Martin Mayora-Neto³, Nigel J. Temperton³, Donato Zipeto¹, Alessandra Ruggiero¹

¹Department of Neuroscience, Biomedicine and Movement, University of Verona, ²Laboratory of Molecular Neurovirology, Faculty of Health Science, University of Brasília, ³Viral Pseudotype Unit, Medway School of Pharmacy, Universities of Kent and Greenwich at Medway

Pseudotyped viruses (PVs) are replication-defective virions that are used to study host-virus interactions under safer conditions than handling authentic viruses. Presented here is a detailed protocol that shows how SARS-CoV-2 PVs can be used to test the neutralizing ability of patients' serum after COVID-19 vaccination.