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Midwestern University/Chicago College of Osteopathic Medicine

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

Method for High Speed Stretch Injury of Human Induced Pluripotent Stem Cell-derived Neurons in a 96-well Format

Jack K. Phillips¹, Sydney A. Sherman^1,2, Sevan R. Oungoulian³, John D. Finan¹

¹Department of Neurosurgery, NorthShore University HealthSystem, ²Midwestern University/Chicago College of Osteopathic Medicine, ³Independent Contractor

Here we present a method for a human in vitro model of stretch injury in a 96-well format on a timescale relevant to impact trauma. This includes methods for fabricating stretchable plates, quantifying the mechanical insult, culturing and injuring cells, imaging, and high content analysis to quantify injury.