Method for High Speed Stretch Injury of Human Induced Pluripotent Stem Cell-derived Neurons in a 96-well FormatJack K. Phillips 1, Sydney A. Sherman 1,2, Sevan R. Oungoulian 3, John D. Finan 1
1Department of Neurosurgery, NorthShore University HealthSystem, 2Midwestern University/Chicago College of Osteopathic Medicine, 3Independent Contractor
Here we present a method for a human in vitro model of stretch injury in a 96-well format on a timescale relevant to impact trauma. This includes methods for fabricating stretchable plates, quantifying the mechanical insult, culturing and injuring cells, imaging, and high content analysis to quantify injury.
