Atlanta VAMC
2 ARTICLES PUBLISHED IN JoVE
Immunology and Infection
Pseudomonas aeruginosa Induced Lung Injury Model
Varsha Suresh Kumar 1, Ruxana T. Sadikot 2, Jeanette E. Purcell 3, Asrar B. Malik 1, Yuru Liu 1
1Department of Pharmacology, University of Illinois College of Medicine, University of Illinois at Chicago, 2Section of Pulmonary and Critical Care Medicine, Atlanta VAMC, Emory University, 3Biologic Resources Lab, University of Illinois at Chicago
We have developed a mouse lung injury model by intra-tracheal injection of bacteria Pseudomonas aeruginosa. This model mimics lung injury during pneumonia and is clinically relevant.
Genetics
Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury
Zhihong Yuan 1,2, Brahmchetna Bedi 1, Ruxana T. Sadikot 1,2
1Department of Veterans Affairs, Atlanta VAMC, 2Division of Pulmonary and Critical Care Medicine, Department of Medicine, Emory University
Mice exposed to intraperitoneal LPS secrete exosomes in Broncho-alveolar lavage (BAL) fluid that are packaged with miRNAs. Using a co-culture system, we show that exosomes released in the BAL fluid disrupt expression of tight junction proteins in bronchial epithelial cells and increase expression of pro-inflammatory cytokines that accentuate lung injury.
JoVE Hakkında
Telif Hakkı © 2020 MyJove Corporation. Tüm hakları saklıdır