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Atlanta VAMC

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Pseudomonas aeruginosa Induced Lung Injury Model

Varsha Suresh Kumar¹, Ruxana T. Sadikot², Jeanette E. Purcell³, Asrar B. Malik¹, Yuru Liu¹

¹Department of Pharmacology, University of Illinois College of Medicine, University of Illinois at Chicago, ²Section of Pulmonary and Critical Care Medicine, Atlanta VAMC, Emory University, ³Biologic Resources Lab, University of Illinois at Chicago

We have developed a mouse lung injury model by intra-tracheal injection of bacteria Pseudomonas aeruginosa. This model mimics lung injury during pneumonia and is clinically relevant.

Genetics

Bronchoalveolar Lavage Exosomes in Lipopolysaccharide-induced Septic Lung Injury

Zhihong Yuan^1,2, Brahmchetna Bedi¹, Ruxana T. Sadikot^1,2

¹Department of Veterans Affairs, Atlanta VAMC, ²Division of Pulmonary and Critical Care Medicine, Department of Medicine, Emory University

Mice exposed to intraperitoneal LPS secrete exosomes in Broncho-alveolar lavage (BAL) fluid that are packaged with miRNAs. Using a co-culture system, we show that exosomes released in the BAL fluid disrupt expression of tight junction proteins in bronchial epithelial cells and increase expression of pro-inflammatory cytokines that accentuate lung injury.