Portland Veterans Affairs Medical Center
2 ARTICLES PUBLISHED IN JoVE
Medicine
Normothermic Cardiac Arrest and Cardiopulmonary Resuscitation: A Mouse Model of Ischemia-Reperfusion Injury
Michael P. Hutchens 1, Richard J. Traystman 2, Tetsuhiro Fujiyoshi 1, Shin Nakayama 1, Paco S. Herson 1
1Department of Anesthesiology and Perioperative Medicine, Oregon Health & Sciences University, 2Department of Pharmacology, University of Colorado Denver
A powerful model for perioperative and critical care related acute kidney injury is presented. Using whole body hypoperfusion induced by cardiac arrest it is possible to nearly replicate the histologic and functional changes of clinical AKI.
Biology
Micropuncture of Bowman's Space in Mice Facilitated by 2 Photon Microscopy
Katsuyuki Matsushita 1, Kirsti Golgotiu 1, Daniel J. Orton 2, Richard D. Smith 2, Karin D. Rodland 2, Paul D. Piehowski 2, Michael P. Hutchens 1,3
1Anesthesiology & Perioperative Medicine, Oregon Health & Science University, 2Environmental and Biological Services Division, Pacific Northwest National Laboratory, 3Operative Care Division, Portland Veterans Affairs Medical Center
We present use of 2-photon microscopy to place a micropipette within Bowman's urinary space in mice, combining 2 foundational techniques of renal physiology. Use of 2-photon microscopy overcomes critical limitations of conventional microscopy for micropuncture renal physiology studies.
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