Portland Veterans Affairs Medical Center

2 ARTICLES PUBLISHED IN JoVE

Medicine

Normothermic Cardiac Arrest and Cardiopulmonary Resuscitation: A Mouse Model of Ischemia-Reperfusion Injury

Michael P. Hutchens¹, Richard J. Traystman², Tetsuhiro Fujiyoshi¹, Shin Nakayama¹, Paco S. Herson¹

¹Department of Anesthesiology and Perioperative Medicine, Oregon Health & Sciences University, ²Department of Pharmacology, University of Colorado Denver

A powerful model for perioperative and critical care related acute kidney injury is presented. Using whole body hypoperfusion induced by cardiac arrest it is possible to nearly replicate the histologic and functional changes of clinical AKI.

Biology

Micropuncture of Bowman's Space in Mice Facilitated by 2 Photon Microscopy

Katsuyuki Matsushita¹, Kirsti Golgotiu¹, Daniel J. Orton², Richard D. Smith², Karin D. Rodland², Paul D. Piehowski², Michael P. Hutchens^1,3

¹Anesthesiology & Perioperative Medicine, Oregon Health & Science University, ²Environmental and Biological Services Division, Pacific Northwest National Laboratory, ³Operative Care Division, Portland Veterans Affairs Medical Center

We present use of 2-photon microscopy to place a micropipette within Bowman's urinary space in mice, combining 2 foundational techniques of renal physiology. Use of 2-photon microscopy overcomes critical limitations of conventional microscopy for micropuncture renal physiology studies.