Isolation of F1-ATPase from the Parasitic Protist Trypanosoma bruceiOndřej Gahura 1, Alena Zíková 1,2
1Biology Centre, Czech Academy of Science, Institute of Parasitology, 2Faculty of Science, University of South Bohemia
This protocol describes the purification of F1-ATPase from the cultured insect stage of Trypanosoma brucei. The procedure yields a highly pure, homogeneous, and active complex suitable for structural and enzymatic studies.
