Accedi

Czech Academy of Science, Institute of Parasitology

1 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Isolation of F₁-ATPase from the Parasitic Protist Trypanosoma brucei

¹Biology Centre, Czech Academy of Science, Institute of Parasitology, ²Faculty of Science, University of South Bohemia

This protocol describes the purification of F₁-ATPase from the cultured insect stage of Trypanosoma brucei. The procedure yields a highly pure, homogeneous, and active complex suitable for structural and enzymatic studies.