The MUB40 Peptide for Use in Detecting Neutrophil-Mediated Inflammation EventsMark C. Anderson 1,2, Louise Injarabian 1,2,3, Antonin Andre 1,2,3, Regis Tournebize 1,2,4, Benoit S. Marteyn 1,2
1Unité de Pathogénie Microbienne Moléculaire, Institut Pasteur, 2INSERM Unité 1202, Institut Pasteur, 3Institut de Biochimie et Génétique Cellulaires, Université Bordeaux Segalen, 4Unit of Technology and Service Photonic BioImaging, Institut Pasteur
Here, we present a protocol to detect the presence of neutrophils in fixed/permeabilized histology sections and assess the activation state of live purified neutrophils. In particular, the MUB40 peptide binds lactoferrin present in neutrophil-specific and tertiary granules. Exposure of the granule contents through either permeabilization or neutrophil activation allows for the marking of neutrophils.
