Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA TranslationHazuki Takahashi *1, Harshita Sharma *1, Piero Carninci 1,2
1Laboratory for Transcriptome Technology, RIKEN Center for Integrative Medical Sciences, 2TransSINE Technologies Co. Ltd.
SINEUPs are synthetic antisense non-coding RNAs, which contain a binding domain (BD) and an effector domain (ED) and up-regulate translation of target mRNA. Here, we describe detection methods for SINEUPs in cultured cell lines, analysis of their translation-promoting activity by Western-blot and a semi-automated high throughput imaging system.
