Beijing Advanced Innovation Center for Structural Biology
2 ARTICLES PUBLISHED IN JoVE
Cancer Research
Establishing Cell Lines Overexpressing DR3 to Assess the Apoptotic Response to Anti-mitotic Therapeutics
Xin Wang *1, Jiamin Zhou *2, Chen Qi *1, Gelin Wang 1,3
1School of Pharmaceutical Sciences, Tsinghua University, 2Department of Radiology, University of California, San Francisco, 3Beijing Advanced Innovation Center for Structural Biology
Establishing a stable cell line overexpressing a gene of interest to study gene function can be done by stable transfection-picking single clones after transfecting them via retroviral infection. Here we show that HT29-DR3 cell lines generated in this way elucidate the mechanisms by which death receptor 3 (DR3) contributes to antimitotics-induced apoptosis.
Biology
Enhancing Density Maps by Removing the Majority of Particles in Single Particle Cryogenic Electron Microscopy Final Stacks
Mengjia Cai *1, Jianying Zhu *2, Qi Zhang *3,4,5,6, Yu Xu 7, Zuoqiang Shi 2,8, Chenglong Bao 2,8,9, Mingxu Hu 1,5,6
1Institute of Bio-Architecture and Bio-Interactions (IBABI), Shenzhen Medical Academy of Research and Translation, 2Yau Mathematical Sciences Center, 3Key Laboratory of Protein Science (Tsinghua University), Ministry of Education, 4School of Life Science, Tsinghua University, 5Beijing Advanced Innovation Center for Structural Biology, 6Beijing Frontier Research Center for Biological Structure, 7Department of Mathematical Sciences, Tsinghua University, 8Yanqi Lake Beijing Institute of Mathematical Sciences and Applications, 9State Key Laboratory of Membrane Biology, School of Life Science
An advanced particle selection method for cryo-EM, namely CryoSieve, improves density map resolution by removing a majority of particles in final stacks, as demonstrated through its application on a real-world dataset.
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