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Beijing Advanced Innovation Center for Structural Biology

2 ARTICLES PUBLISHED IN JoVE

Cancer Research

Establishing Cell Lines Overexpressing DR3 to Assess the Apoptotic Response to Anti-mitotic Therapeutics

Xin Wang ^*1, Jiamin Zhou^*2, Chen Qi^*1, Gelin Wang^1,3

¹School of Pharmaceutical Sciences, Tsinghua University, ²Department of Radiology, University of California, San Francisco, ³Beijing Advanced Innovation Center for Structural Biology

Establishing a stable cell line overexpressing a gene of interest to study gene function can be done by stable transfection-picking single clones after transfecting them via retroviral infection. Here we show that HT29-DR3 cell lines generated in this way elucidate the mechanisms by which death receptor 3 (DR3) contributes to antimitotics-induced apoptosis.

Biology

Enhancing Density Maps by Removing the Majority of Particles in Single Particle Cryogenic Electron Microscopy Final Stacks

Mengjia Cai^*1, Jianying Zhu^*2, Qi Zhang^*3,4,5,6, Yu Xu⁷, Zuoqiang Shi^2,8, Chenglong Bao^2,8,9, Mingxu Hu^1,5,6

¹Institute of Bio-Architecture and Bio-Interactions (IBABI), Shenzhen Medical Academy of Research and Translation, ²Yau Mathematical Sciences Center, ³Key Laboratory of Protein Science (Tsinghua University), Ministry of Education, ⁴School of Life Science, Tsinghua University, ⁵Beijing Advanced Innovation Center for Structural Biology, ⁶Beijing Frontier Research Center for Biological Structure, ⁷Department of Mathematical Sciences, Tsinghua University, ⁸Yanqi Lake Beijing Institute of Mathematical Sciences and Applications, ⁹State Key Laboratory of Membrane Biology, School of Life Science

An advanced particle selection method for cryo-EM, namely CryoSieve, improves density map resolution by removing a majority of particles in final stacks, as demonstrated through its application on a real-world dataset.