Cedars Sinai Medical Center
2 ARTICLES PUBLISHED IN JoVE
Biochemistry
Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion
Miroslava Stastna 1,2,3, Amandine Thomas 1, Juliana Germano 1, Somayeh Pourpirali 1, Jennifer E. Van Eyk 1,2, Roberta A. Gottlieb 1
1The Smidt Heart Institute, Cedars-Sinai Medical Center, 2Advanced Clinical Biosystems Research Institute, Cedars-Sinai Medical Center, 3Institute of Analytical Chemistry of the Czech Academy of Sciences
Here we present a protocol to perform polysome profiling on the isolated perfused mouse heart. We describe methods for heart perfusion, polysome profiling, and analysis of the polysome fractions with respect to mRNAs, miRNAs, and the polysome proteome.
Biochemistry
A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation
Qin Fu 1, Casey W. Johnson 1, Bhagya K. Wijayawardena 2, Michael P. Kowalski 2, Miranda Kheradmand 2, Jennifer E. Van Eyk 1
1Advanced Clinical Biosystems Institute, Smidt Heart institute, Cedars Sinai Medical Center, 2Beckman Coulter Life Sciences
Here, we present an automated plasma protein digestion method for mass spectrometry-based quantitative proteomic analysis. In this protocol, the liquid transferring and incubation steps for protein denaturation, reduction, alkylation, and trypsin digestion reactions are streamlined and automated. It takes approximately five hours to prepare a 96-well plate with desired precision.
JoVE Hakkında
Telif Hakkı © 2020 MyJove Corporation. Tüm hakları saklıdır