A Plasma Sample Preparation for Mass Spectrometry using an Automated WorkstationQin Fu 1, Casey W. Johnson 1, Bhagya K. Wijayawardena 2, Michael P. Kowalski 2, Miranda Kheradmand 2, Jennifer E. Van Eyk 1
1Advanced Clinical Biosystems Institute, Smidt Heart institute, Cedars Sinai Medical Center, 2Beckman Coulter Life Sciences
Here, we present an automated plasma protein digestion method for mass spectrometry-based quantitative proteomic analysis. In this protocol, the liquid transferring and incubation steps for protein denaturation, reduction, alkylation, and trypsin digestion reactions are streamlined and automated. It takes approximately five hours to prepare a 96-well plate with desired precision.
