An Antibody Feeding Approach to Study Glutamate Receptor Trafficking in Dissociated Primary Hippocampal CulturesAndrew M. Chiu 1, Levi Barse 1, Pavla Hubalkova 1,2, Antonio Sanz-Clemente 1
1Department of Pharmacology, Feinberg School of Medicine, Northwestern University, 2Department of Cellular Neurophysiology, Institute of Physiology CAS
This article presents a method to study glutamate receptor (GluR) trafficking in dissociated primary hippocampal cultures. Using an antibody-feeding approach to label endogenous or overexpressed receptors in combination with pharmacological approaches, this method allows for the identification of molecular mechanisms regulating GluR surface expression by modulating internalization or recycling processes.
