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Swiss Federal Institute of Technology Lausanne (EPFL)

2 ARTICLES PUBLISHED IN JoVE

Biology

Measurement of Mitochondrial Mass and Membrane Potential in Hematopoietic Stem Cells and T-cells by Flow Cytometry

Mukul Girotra^*1,2, Anne-Christine Thierry³, Alexandre Harari^1,3, George Coukos¹, Olaia Naveiras^2,4, Nicola Vannini^*1

¹Department of Oncology UNIL CHUV, Ludwig Institute for Cancer Research Lausanne, University of Lausanne, ²Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology Lausanne (EPFL), ³Center of Experimental Therapeutics, Department of Oncology, Centre Hospitalier Universitaire Vaudois, ⁴Hematology Service, Department of Oncology, Centre Hospitalier Universitaire Vaudois (CHUV)

Here we describe a reliable method to measure mitochondrial mass and membrane potential in ex vivo cultured hematopoietic stem cells and T cells.

Biology

Monitoring On-Target Signaling Responses in Larval Zebrafish - Z-REX Unmasks Precise Mechanisms of Electrophilic Drugs and Metabolites

Kuan-Ting Huang¹, Phillippe Ly¹, Jesse R. Poganik², Saba Parvez³, Marcus J. C. Long⁴, Yimon Aye¹

¹Swiss Federal Institute of Technology Lausanne (EPFL), ²Division of Genetics, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, ³Department of Pharmacology and Toxicology, College of Pharmacy, University of Utah, ⁴University of Lausanne (UNIL)

Zebrafish targeting reactive electrophiles and oxidants (Z-REX) is a chemical biology-based method for the investigation of reactive small-molecule signaling. This technique can be applied to live fish of different developmental stages. Here, we couple standard assays in zebrafish with Z-REX for signaling pathway analysis.