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Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg and Universitätsklinikum Erlangen

2 ARTICLES PUBLISHED IN JoVE

Cancer Research

Isolation of Human Endothelial Cells from Normal Colon and Colorectal Carcinoma - An Improved Protocol

Elisabeth Naschberger¹, Daniela Regensburger¹, Clara Tenkerian¹, Melanie Langheinrich², Felix B. Engel³, Carol Geppert⁴, Arndt Hartmann⁴, Robert Grützmann², Vera S. Schellerer^*2, Michael Stürzl^*1

¹Division of Molecular and Experimental Surgery, Department of Surgery, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg, ²Department of Surgery, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg, ³Division of Nephropathology, Department of Pathology, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg, ⁴Department of Pathology, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg

Tumor endothelial cells are important determinants of the tumor microenvironment and the course of the disease. Here, a protocol for the isolation of pure and viable endothelial cells from human colorectal carcinoma and normal colon to be used in drug testing and pathogenesis research is described.

Medicine

Investigating Intestinal Barrier Breakdown in Living Organoids

Marco Bardenbacher¹, Barbara Ruder², Natalie Britzen-Laurent¹, Elisabeth Naschberger¹, Christoph Becker², Ralph Palmisano³, Michael Stürzl^*1, Philipp Tripal^*1,3

¹Division of Molecular and Experimental Surgery, Department of Surgery, Translational Research Center, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg and Universitätsklinikum Erlangen, ²Department of Medicine 1, Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg and Universitätsklinikum Erlangen, ³Optical Imaging Centre Erlangen (OICE), Friedrich-Alexander-Universität (FAU) Erlangen-Nürnberg

Here we describe a technique to quantify the barrier integrity of small intestinal organoids. The fact that the method is based on living organoids enables the sequential investigation of different barrier integrity modulating substances or combinations thereof in a time-resolved manner.