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University Medical Center Rostock

4 ARTICLES PUBLISHED IN JoVE

Medicine

Intravital Microscopy and Thrombus Induction in the Earlobe of a Hairless Mouse

Daniel Strüder^*1, Eberhard Grambow^*2, Ernst Klar², Robert Mlynski¹, Brigitte Vollmar³

¹Department of Otorhinolaryngology, Head and Neck Surgery "Otto Koerner", Rostock University Medical Center, ²Department of General, Thoracic, Vascular and Transplantation Surgery, Rostock University Medical Center, ³Institute for Experimental Surgery, Rostock University Medical Center

The ear model of the hairless SKH1-Hr^hr mouse enables intravital fluorescence microscopy of microcirculation and phototoxic thrombus induction without prior surgical preparation in the examined microvascular bed. Therefore, the ear of the hairless mouse is an excellent in vivo model to study the complex interactions during microvascular thrombus formation, thrombus evolution, and thrombolysis.

Medicine

Synergizing Antegrade Endoscopic with Bridging Vein Harvesting for Improvement of Great Saphenous Vein Graft Quality from the Lower Leg

Christian Klopsch¹, Alexander Kaminski¹, Friedrich Prall², Pascal Dohmen^1,3

¹Department of Cardiac Surgery, Heart Center Rostock, Rostock University Medical Center, University of Rostock, ²Institute of Pathology, Rostock University Medical Center, University of Rostock, ³Department of Cardiothoracic Surgery, Faculty of Health Science, University of the Free State

Presented here is a protocol for antegrade endoscopic vein harvesting from the lower leg, which can safely be introduced in routine coronary artery bypass grafting. Vein grafts present excellent graft quality following this standardized protocol with positioning of the legs, minimally invasive access to the vein, and antegrade endoscopic vein harvesting.

Medicine

Intrathecal Application of a Fluorescent Dye for the Identification of Cerebrospinal Fluid Leaks in Cochlear Malformation

Nora M. Weiss¹, Ingo Andus², Armin Schneider³, Sönke Langner⁴, Stefanie Schröder¹, Sebastian P. Schraven¹, Robert Mlynski¹

¹Department of Otorhinolaryngology, Head and Neck Surgery, University Medical Center Rostock, ²Rostock University, ³ARRI Medical GmbH, ⁴Department of Radiology and Institute of Diagnostic and Interventional Radiology, University Medical Center Rostock

Intrathecally applied fluorescein is used to achieve intraoperative visualization of CSF leaks. This protocol describes a lumbar puncture, the application of 5% fluorescein, and intraoperative visualization using a fully digital microscope.

Cancer Research

Creation and Maintenance of a Living Biobank - How We Do It

Florian Bürtin¹, Stephanie Matschos², Friedrich Prall³, Christina S. Mullins², Mathias Krohn², Michael Linnebacher²

¹Department of General, Visceral, Vascular and Transplantation Surgery, University Medical Center Rostock, University of Rostock, ²Molecular Oncology and Immunotherapy, Department of General, Visceral, Vascular and Transplantation Surgery, University Medical Center Rostock, ³Institute of Pathology, University Medical Center Rostock

In the following work, we describe the consecutive steps necessary for the establishment of a large biobank of colorectal and pancreatic cancer.