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Institut du Cerveau et de la Moelle Epinière

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO⁺ and Light Sheet Fluorescence Microscopy

Laurent Jacob^*1, Jose Brito^*1,2, Jean-Leon Thomas^1,3

¹Université Pierre et Marie Curie Paris, Sorbonne Université, Institut du Cerveau et de la Moelle Epinière, ²Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, ³Department of Neurology, Yale University School of Medicine

A protocol is presented combining tissue clearing with light sheet fluorescence microscopy (LSFM) to obtain three-dimensional and cellular resolution images of the lymphatic vessels and lymph nodes (LNs) collecting the cerebrospinal fluid (CSF) and spinal epidural fluid.