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Clinical College of Nanjing Medical University

6 ARTICLES PUBLISHED IN JoVE

Biology

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Tyler M. Bauman^1,2, Emily A. Ricke², Sally A. Drew³, Wei Huang^3,4, William A. Ricke^2,4

¹Division of Urologic Surgery, Washington University in St. Louis School of Medicine, ²Department of Urology, University of Wisconsin School of Medicine and Public Health, ³Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, ⁴O’Brien Urology Research Center, University of Wisconsin School of Medicine and Public Health

Immunohistochemistry is a powerful lab technique for evaluating protein localization and expression within tissues. Current semi-automated methods for quantitation introduce subjectivity and often create irreproducible results. Herein, we describe methods for multiplexed immunohistochemistry and objective quantitation of protein expression and co-localization using multispectral imaging.

Biochemistry

High Throughput, Absolute Determination of the Content of a Selected Protein at Tissue Levels Using Quantitative Dot Blot Analysis (QDB)

Xiaoying Qi^*1, Yunyun Zhang^*2, Yuan Zhang¹, Tianhui Ni³, Wenfeng Zhang², Chunhua Yang¹, Jia Mi^1,4, Jiandi Zhang^2,3, Geng Tian¹

¹Medicine and Pharmacy Research Center, Binzhou Medical University, ²Yantai Zestern Biotechnique Co. LTD, ³Precision Medicine research center, Binzhou Medical University, ⁴Department of Chemistry - BMC, Uppsala University

Here we demonstrate a detailed process of quantitative dot blot analysis (QDB) by determining the absolute content of a targeted protein, capping actin protein, gelsolin-like (CAPG), in three different mouse tissues. We demonstrate a high throughput, convenient, quantitative immunoblot technique for biomarker validation at the cellular and tissue level.

Engineering

Sandy Soil Improvement through Microbially Induced Calcite Precipitation (MICP) by Immersion

Shihui Liu¹, Kang Du¹, Kejun Wen¹, Wei Huang², Farshad Amini¹, Lin Li³

¹Department of Civil & Environmental Engineering, Jackson State University, ²School of Civil Engineering and Architecture, Chongqing University of Science and Technology, ³Department of Civil & Architectural Engineering, Tennessee State University

Here, microbially induced calcite precipitation (MICP) technology is presented to improve soil properties by immersion.

Medicine

Establishment of a Severe Dry Eye Model Using Complete Dacryoadenectomy in Rabbits

Robert A. Honkanen¹, Liqun Huang^2,3, Wei Huang¹, Basil Rigas^2,4

¹Department of Ophthalmology, Stony Brook University, ²Department of Medicine, Stony Brook University, ³Medicon Pharmaceuticals, Inc, ⁴Department of Preventive Medicine, Stony Brook University

A novel approach is presented to induce chronic dry eye disease in rabbits by surgically removing all orbital lacrimal glands. This method, distinct from those previously reported, produces a stable, reproducible model of aqueous deficient dry eye well suited to study tear physiology and pathophysiology and ocular therapeutics.

Medicine

A Doxorubicin-Induced Murine Model of Dilated Cardiomyopathy In Vivo

Yihai Liu¹, Wenfeng Zhang¹, Tingting Hu¹, Jie Ni¹, Biao Xu¹, Wei Huang¹

¹Department of Cardiology, Nanjing Drum Tower Hospital, Clinical College of Nanjing Medical University

Described is a protocol to establish a Doxorubicin-induced dilated cardiomyopathy (DCM) model in mice via long-term intraperitoneal injection of Doxorubicin.

Medicine

Real-Time Dynamic Navigation System for the Precise Quad-Zygomatic Implant Placement in a Patient with a Severely Atrophic Maxilla

Yihan Shen^*1, Qinggang Dai^*1, Baoxin Tao^*1, Wei Huang², Feng Wang², Kengliang Lan¹, Yuanyuan Sun¹, Xiaowan Ling¹, Lijun Yan¹, Yueping Wang¹, Yiqun Wu¹

¹The 2nd Dental Center, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, ²Department of Dental Implantology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology

Here, we present a protocol to achieve precise quad-zygomatic implant placement in patients with severely atrophic maxilla using a real-time dynamic navigation system.