Three-Dimensional Motor Nerve Organoid GenerationTatsuya Osaki 1,2, Siu Yu A. Chow 1,2, Yui Nakanishi 1,2, Joel Hernández 1,3, Jiro Kawada 4, Teruo Fujii 1, Yoshiho Ikeuchi 1,2
1Institute of Industrial Science, The University of Tokyo, 2Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 3Faculty of Science and Engineering, Tecnologico de Monterrey, 4Jiksak Bioengineering, Inc.
This protocol provides a comprehensive procedure to fabricate human iPS cell-derived motor nerve organoid through spontaneous assembly of a robust bundle of axons extended from a spheroid in a tissue culture chip.
