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The General Hospital of Western Theater Command

2 ARTICLES PUBLISHED IN JoVE

Cancer Research

Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8⁺ T Cells in Mice

Lisha Wang¹, Zhiming Wang¹, Junyi Guo², Huayu Lin¹, Shuqiong Wen², Qiao Liu¹, Yiding Li³, Qing Wu¹, Leiqiong Gao¹, Xiangyu Chen¹, Luoyingzi Xie¹, Qin Tian¹, Jianfang Tang¹, Zhirong Li¹, Li Hu¹, Juan Wang⁴, Lifan Xu¹, Qizhao Huang⁵, Lilin Ye¹

¹Institute of Immunology, Third Military Medical University, ²Guanghua School of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Stomatological Hospital, Sun Yat-Sen University, ³Shigatse Branch, Xinqiao Hospital, Third Military Medical University, ⁴Department of Emergency Medicine, Southwest Hospital, Third Military Medical University, ⁵Cancer Center, The General Hospital of Western Theater Command

Here, we present a tumor transplantation protocol for the characterization of tumor-inherent and periphery-derived tumor-infiltrated lymphocytes in a mouse tumor model. Specific tracing of the influx of recipient-derived immune cells with flow cytometry reveals the dynamics of the phenotypic and functional changes of these cells during antitumor immune responses.

Medicine

Acupuncture as a Therapeutic Intervention for Acute Gastrointestinal Injury (AGI): A Preliminary Study

Peng Ding^*1, Yuan Zhou^*2, Xiujuan Zhou^*1, Weiting Sun¹, Peiyang Gao¹

¹Department of Critical Care Medicine, Hospital of Chengdu University of Traditional Chinese Medicine, ²Department of Geriatrics, The General Hospital of Western Theater Command

Acute gastrointestinal injury (AGI) is a prevalent occurrence in intensive care units and has been linked to unfavorable patient outcomes. The current approaches focus on addressing the primary disease and providing symptomatic relief. The protocol here demonstrates the implementation of acupuncture as a therapeutic intervention for AGI.