Ectopic Tumor Implantation of B16F10-OVA Cells in the Inguinal Region of Mice
2:23
Adoptive Transfer of Congenically Marked OT-I T Cells into Tumor-Bearing Mice
3:26
Dissect Tumor Mass from Tumor-Bearing Donor Mice
4:31
Subcutaneous Transplantation of Donor-Derived Tumor onto the Tumor-Matched Recipient Mice
6:14
Results: For Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice
7:04
Conclusion
Transkript
This method can help answer the key questions in tumor immunology, including whether there is a replenishment of tumor-specific immune cells from peripheral tissues, the transitions between newly infiltrated and already existing immune cells withi
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Here, we present a tumor transplantation protocol for the characterization of tumor-inherent and periphery-derived tumor-infiltrated lymphocytes in a mouse tumor model. Specific tracing of the influx of recipient-derived immune cells with flow cytometry reveals the dynamics of the phenotypic and functional changes of these cells during antitumor immune responses.