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Affiliated Hospital of Jiangsu University

3 ARTICLES PUBLISHED IN JoVE

Biology

Real-time Analyses of Retinol Transport by the Membrane Receptor of Plasma Retinol Binding Protein

¹Department of Physiology, Jules Stein Eye Institute and Howard Hughes Medical Institute, University of California, Los Angeles

Here we describe an optimized technique to produce high-quality vitamin A/RBP complex and two real-time monitoring techniques to study vitamin A transport by STRA6, the RBP receptor.

Medicine

Intra-Operative Neural Monitoring of Thyroid Surgery in a Porcine Model

Che-Wei Wu^1,2,3, Tzu-Yen Huang², Hui-Chun Chen⁴, Hsiu-Ya Chen⁵, Tsung-Yi Tsai², Pi-Ying Chang⁵, Yi-Chu Lin², Hsin-Yi Tseng², Pao-Chu Hun⁶, Xiaoli Liu⁷, Hui Sun⁷, Gregory W. Randolph⁸, Gianlorenzo Dionigi⁹, Feng-Yu Chiang^2,3, I-Cheng Lu^5,10,11

This study aims to develop a standard protocol of intra-operative neural monitoring of thyroid surgery in a porcine model. Here, we present a protocol to demonstrate general anesthesia, to compare different types of electrodes, and to investigate the electrophysiological characteristics of the normal and injured recurrent laryngeal nerves.

JoVE Core

Direct Reprogramming of Mouse Fibroblasts into Melanocytes

Yi-Xuan Zhang^1,2, Li-Ping Liu^1,2, Ming Jin^1,2, Hui Sun^1,2, Han-Lin Zhang^1,2, Yu-Mei Li^1,2

¹Institute for Regenerative Medicine, Jiangsu University, ²Department of Dermatology, Affiliated Hospital of Jiangsu University

Here, we describe an optimized direct reprogramming system for melanocytes and a high-efficiency, concentrated virus packaging system that ensures smooth direct reprogramming.