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Medical College of Wisconsin & Marquette University

4 ARTICLES PUBLISHED IN JoVE

Bioengineering

Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles

Brandon J. Tefft¹, Susheil Uthamaraj², J. Jonathan Harburn³, Martin Klabusay⁴, Dan Dragomir-Daescu^2,5, Gurpreet S. Sandhu¹

¹Division of Cardiovascular Diseases, Mayo Clinic, ²Division of Engineering, Mayo Clinic, ³School of Medicine, Pharmacy and Health, Durham University, ⁴Regional Center for Applied Molecular Oncology, Masaryk Memorial Cancer Institute, ⁵Mayo Clinic College of Medicine, Mayo Clinic

Targeted cell delivery is useful in a variety of biomedical applications. The goal of this protocol is to use superparamagnetic iron oxide nanoparticles (SPION) to label cells and thereby enable magnetic cell targeting approaches for a high degree of control over cell delivery and localization.

Bioengineering

Ferromagnetic Bare Metal Stent for Endothelial Cell Capture and Retention

Susheil Uthamaraj¹, Brandon J. Tefft², Ota Hlinomaz³, Gurpreet S. Sandhu², Dan Dragomir-Daescu^1,4

¹Division of Engineering, Mayo Clinic, ²Division of Cardiovascular Diseases, Mayo Clinic, ³Department of Cardioangiology, ICRC, St. Anne's University Hospital, ⁴Mayo Clinic College of Medicine

Our goals were to design, manufacture and test ferromagnetic stents for endothelial cell capture. Ten stents were tested for fracture and 10 more stents were tested for retained magnetism. Finally, 10 stents were tested in-vitro and 8 more stents were implanted in 4 pigs to show cell capture and retention.

Medicine

Fabrication of Small Caliber Stent-grafts Using Electrospinning and Balloon Expandable Bare Metal Stents

Susheil Uthamaraj¹, Brandon J. Tefft², Soumen Jana², Ota Hlinomaz³, Manju Kalra⁴, Amir Lerman², Dan Dragomir-Daescu^1,5, Gurpreet S. Sandhu²

¹Division of Engineering, Mayo Clinic, ²Department of Cardiovascular Diseases, Mayo Clinic, ³Department of Cardioangiology, ICRC, St. Anne's University Hospital, ⁴Department of Vascular Surgery, Mayo Clinic, ⁵Department of Physiology and Biomedical Engineering, Mayo Clinic

In the protocol, we present a method to manufacture a small caliber stent-graft by sandwiching a balloon expandable stent between two electrospun nanofibrous polyurethane layers.

Bioengineering

Characterization of Blood Outgrowth Endothelial Cells (BOEC) from Porcine Peripheral Blood

Akankshya Shradhanjali¹, Susheil Uthamaraj², Dan Dragomir-Daescu³, Rajiv Gulati⁴, Gurpreet S. Sandhu⁴, Brandon J. Tefft¹

¹Department of Biomedical Engineering, Medical College of Wisconsin & Marquette University, ²Division of Engineering, Mayo Clinic, ³Department of Physiology & Biomedical Engineering, Mayo Clinic, ⁴Department of Cardiovascular Medicine, Mayo Clinic

Characterization of Blood Outgrowth Endothelial Cells (BOEC) from Porcine Peripheral Blood