Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering MicroscopyAlejandro De la Cadena 1, Federico Vernuccio 1, Benedetta Talone 1, Arianna Bresci 1, Chiara Ceconello 1, Subir Das 1, Renzo Vanna 2, Giulio Cerullo 1,2, Dario Polli 1,2
1Dipartimento di Fisica, Politecnico di Milano, 2Institute for Photonics and Nanotechnologies (IFN-CNR)
We present a protocol to acquire chemical images with broadband stimulated Raman scattering (SRS) microscopy. Based on an SRS microscope that operates with differential multichannel-lock-in detection, the protocol describes the sample preparation, adjustment of the SRS apparatus, and chemometrics to disentangle different constituents of chemically heterogeneous samples.
