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The University of Trans-Disciplinary Health Sciences and Technology

2 ARTICLES PUBLISHED IN JoVE

Developmental Biology

In Ovo and Ex Ovo Methods to Study Avian Inner Ear Development

Nishant Singh^*1,2, Anubhav Prakash^*1, Suraj Ranganath Chakravarthy^*1, Raman Kaushik^*1, Raj K. Ladher¹

¹National Centre for Biological Sciences, Tata Institute of Fundamental Research, ²The University of Trans-Disciplinary Health Sciences and Technology

The chick is a cost-effective, accessible, and widely available model organism for a variety of studies. Here, a series of protocols is detailed to understand the molecular mechanisms underlying avian inner ear development and regeneration.

Behavior

Tracking Sugar-Elicited Local Searching Behavior in Drosophila

Manal Shakeel^1,2, Shivam Kaushik³, Teiichi Tanimura⁴, Axel Brockmann¹, Pinky Kain^3,5

¹National Centre for Biological Sciences, ²The University of Trans-Disciplinary Health Sciences and Technology, ³Regional Centre for Biotechnology, ⁴Neural Circuit Group, Division of Biological Science, Graduate School of Science, Nagoya University, ⁵Perelman School of Medicine, University of Pennsylvania

This protocol describes a behavioral assay for recording sugar-elicited search behavior using Drosophila melanogaster. The assay can be utilized to study feeding and foraging-related behaviors, as well as the underlying neuronal mechanisms.