This material is based upon work supported by the U.S. Department of Homeland Security under Grant Award Number 2010-ST-061-FD0001 through a grant awarded by the National Center for Food Protection and Defense at the University of Minnesota. Disclaimer: The views and conclusions contained in this document are those of the authors and should not be interpreted as necessarily representing the official policies, either expressed or implied, of the U.S. Department of Homeland Security or the National Center for Food Protection and Defense.

1. Gümüş Nanoparçacık Sentezi 15 <ol><li> 5 saniye boyunca 100 mi, aşırı saf su (18.2 ΩU) ve vorteks 18 mg gümüş nitrat çözeltisi ile çözülmüştür. </li><li> 5 saniye boyunca 1 ml su ve vorteks 27 mg sodyum sitrat dihidrat çözülür. </li><li> Bir karıştırma çubuğu ihtiva eden bir erlene hazırlanan gümüş nitrat çözeltisi her aktarın ve manyetik bir sıcak plaka üzerinde akıtma koydu. ~ 350 ° C de, 700 rpm&#39;lik bir karıştırma hızı ile kuvvetli karıştırma altında balon Isı (plaka sıcaklık ayarı). </li><li> kaynar zaman hemen erlene hazırlanan sodyum sitrat çözeltisi tüm ekleyin ve çözüm Ag NPS oluşumunu gösteren yeşilimsi kahverengi, açılana kadar ilave 25 dakika kaynatın çözüm bırakın. </li><li> Sıcak plaka şişeyi çıkarın ve sabit renk ve tran ile, başka bir manyetik plaka üzerine koydu (ısı değil) ve heyecan O / N oda sıcaklığında aynı karıştırma hızında karışım istikrarlı bir duruma ulaşana kadarsparency. Gerekirse hazırlanan Ag NPlerin emişi belirlemek üzere bir UV spektrometresi kullanarak. </li><li> 100 ml saf su ile nihai karışımı ile seyreltilir. </li><li> Gerekirse, üreticinin protokolüne uygun olarak Ag NPlerin boyutunu ölçmek için, bir Zetasizer kullanarak. </li><li> kapalı bir kaba Ag kolloid aktarın ve alüminyum folyo ile ışıktan korumak. Gerekirse kolloid 2 ay boyunca 4-7 ° C&#39;de buzdolabında saklanır. </li></ol> Bir SERS Aktif Filtre Membran 2. Fabrikasyon <ol><li> 50 mM NaCI solüsyonu yapmak üzere, 100 ml su içinde 2.92 g sodyum klorür (NaCI) içinde çözülür. </li><li> hazırlanan Ag NPlerin 1 ml 5 mM NaCI çözeltisi 1 ml ilave edilir ve 20 rpm&#39;de 10 dakika için bir sallanan karıştırıcıda karıştırın. Bu adım Ag nanoclustere içine Ag NPS bir araya etmektir. </li><li> Bir şırıngaya birleştirilebilen bir filtre tutucusu, bir membran filtre (PVDF, 0.1 um gözenek boyutu) yerleştirin. Daha küçük gözenek boyutu zar fo oldututarlı sinyalleri Ag nanoyığınlar yakalama ve üretiminde büyük gözenek boyutu membranı (yani, 0.22 mikron) daha und daha etkili. </li><li> Yük filtrasyon için şırınga içine hazırlanmış Ag nanoclustere 2 ml. şırınga filtre yuvasını takın ve elle 1 damla / sn akış hızında zarından Ag nanoclustere tüm hacmini geçmektedir. Membran tuzakları Ag nanokümeler, bir SERS-aktif filtre membran oluşturur. </li><li> Filtre tutucu filtre membran ayırın. zara hiçbir zarar sağlamak için bir cımbız kullanarak dış kenarında zarı tutarken özellikle dikkat gereklidir. Bir bardak slayt yaklaşık 3 dk ve yer zarının hava kuru. </li><li> SERS substratın Raman algılama <ol><li> 5 mW, 10X mikroskobik hedefi ayarlayın 2. 1 sn ve maruziyet numarasının pozlama süresi bir lazer gücüne sahip bir 780 nm dalga boyundaki lazer Raman cihazı ayarlayın. yazılım üzerinde objektif çok buna ayarlanmış olduğundan emin olun. &lt;/ Li&gt; <li> Raman aracın platform üzerine üstündeki membran ile cam slayt yerleştirin ve membran yüzeyinde odaklanmak için mikroskop kullanıyoruz. </li><li> Rasgele membran yüzeyinde 8-10 noktalar seçin ve enstrüman sırayla otomatik olarak toplayacaktır. analiz için üreticinin yazılımı açın spektral verileri. </li></ol></li></ol> SERS Aktif Filtre Sisteminin 3. Uygulama Kimyasal Bulaşanlar Algılama için <ol><li> 10 ppb ferbam çözeltisi hazırlayın. Dikkat: ferbam çok uçucudur. önlemler (maske ve gözlük) katı ağırlığında kullanın. <ol><li> 2 mg ferbam tozu tartılır ve bir stok çözeltisi (100 ppm) yapmak için 20 ml% 50 asetonitril (10 ml asetonitril ve 10 ml su) içinde çözülür. 30 saniye için şişeyi vorteksleyin. </li><li> Bir test tüpü içinde, 100 ppm ferbam çözeltisi 1 ml alın ve 10 ppm bir solüsyon hazırlamak için 9 ml% 50 asetonitril ekleyin. 5 saniye boyunca tüp vorteksleyin. </li><li> 1 ml alın10 ppm test tüpünde çözeltisi ve 9 ml% 50 asetonitril ilave 1 ppm bir solüsyon hazırlamak için. 5 saniye boyunca tüp vorteksleyin. </li><li> Bir test tüpü içinde 1 ppm çözeltisi 1 ml alın ve 100 ppb solüsyon yapmak için 9 ml% 50 asetonitril ekleyin. 5 saniye boyunca tüp vorteksleyin. </li><li> Bir test tüpü içinde, 100 ppb çözeltisi 1 ml alın ve 10 ppb solüsyon yapmak için 9 ml% 50 asetonitril ekleyin. 5 saniye boyunca tüp vorteksleyin. </li></ol></li><li> 1 ppm ampisilin çözeltisi hazırlayın. <ol><li> 10 mg ampisilin toz tartılır ve 100 ppm ampisilin solüsyon yapmak için, 100 ml su içinde çözülür. 30 saniye için şişeyi vorteksleyin. </li><li> Bir test tüpü içinde, 100 ppm çözeltisi 1 ml alın ve 10 ppm ampisilin çözeltisinin yapılması için 9 mL su ilave edilir. 5 saniye boyunca tüp vorteksleyin. </li><li> Bir test tüpü içinde 10 ppm çözeltisi 1 ml alın ve 1 ppm ampisilin çözeltisinin yapılması için 9 mL su ilave edilir. 5 saniye boyunca tüp vorteksleyin. </li></ol></li><li> yukarı bakacak şekilde NP kaplı tarafı, filtre yuvasına geri filtre membran koyun. &lt;/ Li&gt; <li> Yük 5 Yeni enjektöre bir örnek ml ve daha sonra içinde Ag kaplı membran ile filtre yuvasına takın. </li><li> El 1 damla / sn arasında bir akış hızında zar içinden numunenin tüm hacmi geçmektedir. Hedef moleküller, filtre zarı kaplanmış NP üzerine adsorbe edildi ve konsantre edilebilir. </li><li> yaklaşık 3 dakika boyunca filtre tutucu, hava kuru filtre zarı ayırmak ve adım 2.6 açıklandığı gibi aynı yöntemi kullanarak Raman aleti kullanılarak sinyalleri ölçmek. </li><li> 2.6 adımı tekrarlayın 2.2 başka Ag kaplı membran hazırlamak ve diğer numune tespiti için adım 3,3 takip etmek. </li></ol>

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The authors have nothing to disclose.

Bu protokol kritik adımlardan biri üniforma Ag NPS tutarlı sonuçlar için anahtar Ag NPS sentezi vardır. Isıtma süresi ve ön-maddeleri konsantrasyonları kesin olarak kontrol edilmelidir. Bu AGNPS hazırlanması ortalama büyüklüğü Zetasizer ile ölçülmüştür 80 nm, (veri gösterilmemiştir). Diğer önemli adım tuz konsantrasyonu ve toplama zamanı kesin olarak kontrol edilmelidir tuz toplanmasıdır. daha küçük bir gözenek boyutuna sahip membran tuzağı Ag nanoklastırlaar daha etkili bulunmuştu...

Yüzey Raman spektroskopisi (SERS) nanoteknoloji ile Raman spektroskopisi birleştiren bir tekniktir. Asil metalik nano-yüzeylerde analitlerin Raman saçılması yoğunluğu büyük ölçüde lokalize yüzey plazmon rezonansı ile geliştirilmiştir. 1 Gümüş nanopartiküller (Ag NPS) artık farkla en yaygın olarak kullanılan SERS nedeniyle yüksek geliştirme yeteneği yüzeylerde. 2 kadar olan Ag NPlerin çeşitli sentetik yöntemler geliştirilmiştir. 3-6 Ag NPS, sensitivite ve / veya işlevini geliştirmek için etkili bir SERS substratlar olarak tek başına ya da diğer malzemeler ve yapılar ile kombine edilebilir. 7-11 SERS teknikleri, gıda ve çevre örneklerinde çeşitli eser miktarda kirletici tespiti için büyük bir kapasiteye göstermiştir 12 Geleneksel olarak, bir SERS örneği hazırlamak için iki ortak yolu vardır:.. Çözüm tabanlı ve yüzey-temelli yöntemler 13 çözüm tabanlı yöntemiid örnekleri ile karıştırmak için NP kolloidler kullanır. Daha sonra NP-analit kompleksi santrifüj kullanılarak toplandı ve kurutulduktan sonra Raman ölçümü için bir katı destek üzerine tatbik edilir. Alt tabaka esas yöntem genellikle prefabrike bir katı alt-tabaka üzerine bir sıvı örneğinin birçok mikrolitre biriktirilmesi ile uygulanır. 14. Bununla birlikte, bu iki yöntem de, numune hacminin büyük bir miktarda etkin ve uygulanabilir. SERS testlerin bazı değişiklikler böyle bir filtre sistemi 15-21 entegrasyonu veya bir mikroakışkan cihazın dahil olarak hacim sınırları aştı. 21-24 modifiye SERS deneyleri, kimyasal kirleticiler izlemek için duyarlılık ve fizibilite büyük artışı göstermiştir büyük su örneklerinde. Burada pestisit ferbam ve antibiyotik ampisilin kalıntılarını tespit etmek için, imalat ve şırıngalı bir filtre göre SERS yönteminin uygulamanın ayrıntılı protokol göstermektedir.

<table><tbody><tr><td>Ampicillin</td><td>Fisher Scientific</td><td>BP1760-5</td><td>N/A</td></tr><tr><td>Ferbam</td><td>Chem Service</td><td>N-11970-250MG</td><td>98+%</td></tr><tr><td>Silver nitrate</td><td>Sigma Aldrich</td><td>209139</td><td>99.0+%</td></tr><tr><td>Sodium citrate dehydrate</td><td>Sigma Aldrich</td><td>W302600</td><td>99+%</td></tr><tr><td>Sodium chloride</td><td>Sigma Aldrich</td><td>S7653</td><td>99.5+%</td></tr><tr><td>EMD Millipore Durapore PVDF Membrane Filters</td><td>Fisher Scientific</td><td>VVLP01300</td><td>0.10 &amp;micro;m Pore Size, hydrophilic</td></tr><tr><td>Polycarbonate Filter Holders</td><td>Cole-Parmer</td><td>EW-29550-40</td><td>13 mm diameter</td></tr><tr><td>Analog Vortex Mixer</td><td>Fisher Scientific</td><td>02-215-365</td><td>N/A</td></tr><tr><td>Nutating Mixers</td><td>Fisher Scientific</td><td>05-450-213</td><td>N/A</td></tr><tr><td>DXR Raman spectroscope</td><td>Thermo Scientific</td><td>IQLAADGABFFAHCMAPB</td><td>Laser power: 1 mW&lt;br /&gt; Exposure time: 5 sec</td></tr></tbody></table>

a filter-based surface enhanced raman spectroscopic assay for rapid detection of chemical contaminants

We demonstrate a method to fabricate highly sensitive surface-enhanced Raman spectroscopic (SERS) substrates using a filter syringe system that can be applied to the detection of various chemical contaminants. Silver nanoparticles (Ag NPs) are synthesized via reduction of silver nitrate by sodium citrate. Then the NPs are aggregated by sodium chloride to form nanoclusters that could be trapped in the pores of the filter membrane. A syringe is connected to the filter holder, with a filter membrane inside. By loading the nanoclusters into the syringe and passing through the membrane, the liquid goes through the membrane but not the nanoclusters, forming a SERS-active membrane. When testing the analyte, the liquid sample is loaded into the syringe and flowed through the Ag NPs coated membrane. The analyte binds and concentrates on the Ag NPs coated membrane. Then the membrane is detached from the filter holder, air dried and measured by a Raman instrument. Here we present the study of the volume effect of Ag NPs and sample on the detection sensitivity as well as the detection of 10 ppb ferbam and 1 ppm ampicillin using the developed assay.

Bu deneyde önemli adımlar şematik diyagram (Şekil 1) gösterilmiştir. 2 maksimize hassasiyeti ulaşmak için membran kaplama olarak AGNPS optimize hacmini kullanmak için önemini göstermiştir Şekil. ferbam kullanıldığında 0.5 ml (yetersiz kaplama) ya da 2 ml (çok fazla kaplama) ile karşılaştırıldığında, Ag NPS 1 ml güçlü sinyali sağlar. Biz geliştiri...

Watch this Scientific Journal Video about A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants at JoVE.com

A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants

Imalat ve kimyasal kirleticilerin (örneğin, pestisid ferbam ve antibiyotik ampisilin) ​​tespiti için Raman spektroskopi (SERS) deneyi geliştirilmiş filtre tabanlı yüzey yapmak için bir prosedür sunulmuştur.

Kimyasal bulaşanların Hızlı Algılama için Raman Spektroskopisi Tahlili Geliştirilmiş A Filtre tabanlı Yüzey

We demonstrate a method to fabricate highly sensitive surface-enhanced Raman spectroscopic (SERS) substrates using a filter syringe system that can be ...

a-filter-based-surface-enhanced-raman-spectroscopic-assay-for-rapid

Research

JoVE Journal

Chemistry

9.3K Görüntüleme.  University of Massachusetts Amherst.  Imalat ve kimyasal kirleticilerin (örneğin, pestisid ferbam ve antibiyotik ampisilin) ​​tespiti için Raman spektroskopi (SERS) deneyi geliştirilmiş filtre tabanlı yüzey yapmak için bir prosedür sunulmuştur. 

Video: A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants

Fabricating a UV-Vis and Raman Spectroscopy Immunoassay Platform

Immunoassays are used to detect proteins based on the presence of associated antibodies. Because of their extensive use in research and clinical settings, a large infrastructure of immunoassay instruments and materials can be found. For example, 96- and 384-well polystyrene plates are available commercially and have a standard design to accommodate ultraviolet-visible (UV-Vis) spectroscopy machines from various manufacturers. In addition, a wide variety of immunoglobulins, detection tags, and blocking agents for customized immunoassay designs such as enzyme-linked immunosorbent assays (ELISA) are available.
Despite the existing infrastructure, standard ELISA kits do not meet all research needs, requiring individualized immunoassay development, which can be expensive and time-consuming. For example, ELISA kits have low multiplexing (detection of more than one analyte at a time) capabilities as they usually depend on fluorescence or colorimetric methods for detection. Colorimetric and fluorescent-based analyses have limited multiplexing capabilities due to broad spectral peaks. In contrast, Raman spectroscopy-based methods have a much greater capability for multiplexing due to narrow emission peaks. Another advantage of Raman spectroscopy is that Raman reporters experience significantly less photobleaching than fluorescent tags1. Despite the advantages that Raman reporters have over fluorescent and colorimetric tags, protocols to fabricate Raman-based immunoassays are limited. The purpose of this paper is to provide a protocol to prepare functionalized probes to use in conjunction with polystyrene plates for direct detection of analytes by UV-Vis analysis and Raman spectroscopy. This protocol will allow researchers to take a do-it-yourself approach for future multi-analyte detection while capitalizing on pre-established infrastructure.

Nanoparticle-based optical probes have been designed as a vehicle for detecting antigens using Raman and UV-Vis spectroscopy. Here we describe a protocol for preparing such probes for a UV-Vis/Raman spectroscopy immunoassay in such a way to incorporate future multiplexing capabilities.

Bir UV-Vis ve Raman Spektroskopisi İmmunoassay Platformu imalatı

Immunoassays are used to detect proteins based on the presence of associated antibodies. Because of their extensive use in research and clinical settings, ...

Biyokimya

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Fabrication and characterization of conjugate nano-biological systems interfacing metallic nanostructures on solid supports with immobilized biomolecules is reported. The entire sequence of relevant experimental steps is described, involving the fabrication of nanostructured substrates using electron beam lithography, immobilization of biomolecules on the substrates, and their characterization utilizing surface-enhanced Raman spectroscopy (SERS). Three different designs of nano-biological systems are employed, including protein A, glucose binding protein, and a dopamine binding DNA aptamer. In the latter two cases, the binding of respective ligands, D-glucose and dopamine, is also included. The three kinds of biomolecules are immobilized on nanostructured substrates by different methods, and the results of SERS imaging are reported. The capabilities of SERS to detect vibrational modes from surface-immobilized proteins, as well as to capture the protein-ligand and aptamer-ligand binding are demonstrated. The results also illustrate the influence of the surface nanostructure geometry, biomolecules immobilization strategy, Raman activity of the molecules and presence or absence of the ligand binding on the SERS spectra acquired.

We describe the fabrication and characterization of nano-biological systems interfacing nanostructured substrates with immobilized proteins and aptamers. The relevant experimental steps involving lithographic fabrication of nanostructured substrates, bio-functionalization, and surface-enhanced Raman spectroscopy (SERS) characterization, are reported. SERS detection of surface-immobilized proteins, and probing of protein-ligand and aptamer-ligand binding is demonstrated.

Yüzey EBL Fabrikasyon Nanostructured Alt Tabakalar Kullanma biyomoleküllerin Raman Spektroskopisi Algılama Geliştirilmiş

Fabrication and characterization of conjugate nano-biological systems interfacing metallic nanostructures on solid supports with immobilized biomolecules ...

Mühendislik

Preparation and Application of a New Bacterial Biosensor for the Presumptive Detection of Gunshot Residue

MicRoboCop is a biosensor that has been designed for a unique application in forensic chemistry. MicRoboCop is a system made up of three devices that, when used together, can indicate the presence of gunshot residue (GSR) by producing a fluorescence signal in the presence of three key analytes (antimony, lead, and organic components of GSR). The protocol describes the synthesis of the biosensors using Escherichia coli (E. coli), and the analytical chemistry methods used to evaluate the selectivity and sensitivity of the sensors. The functioning of the system is demonstrated by using GSR collected from the inside of a spent cartridge casing. Once prepared, the biosensors can be stored until needed and can be used as a test for these key analytes. A positive response from all three analytes provides a presumptive positive test for GSR, while each individual device has applications for detecting the analytes in other samples (e.g., a detector for lead contamination in drinking water). The main limitation of the system is the time required for a positive signal; future work may involve studying different organisms to optimize the response time.

A protocol is presented using synthetic biology techniques to synthesize a set of bacterial biosensors for the analysis of gunshot residue, and to test the functioning of the devices for their intended use using fluorescence spectroscopy.

Silah kalıntılarının varsayımsal tespiti için yeni bir bakteriyel biyosensörün hazırlanması ve uygulanması

MicRoboCop is a biosensor that has been designed for a unique application in forensic chemistry. MicRoboCop is a system made up of three devices that, ...

Kimya

Multiplex Chemical Imaging Based on Broadband Stimulated Raman Scattering Microscopy

Stimulated Raman scattering (SRS) microscopy is a nonlinear optical technique for label-free chemical imaging. This analytical tool delivers chemical maps at high speed, and high spatial resolution of thin samples by directly interrogating their molecular vibrations. In its standard implementation, SRS microscopy is narrowband and forms images with only a single vibrational frequency at a time. However, this approach not only hinders the chemical specificity of SRS but also neglects the wealth of information encoded within vibrational spectra. 
These limitations can be overcome by broadband SRS, an implementation capable of extracting a vibrational spectrum per pixel of the image in parallel. This delivers hyperspectral data that, when coupled with chemometric analysis, maximizes the amount of information retrieved from the specimen. Thus, broadband SRS improves the chemical specificity of the system, allowing the quantitative determination of the concentration of the different constituents of a sample. Here, we report a protocol for chemical imaging with broadband SRS microscopy, based on a home-built SRS microscope operating with a custom differential multichannel-lock-in amplifier detection. It discusses the sample preparation, alignment of the SRS apparatus, and chemometric analysis. By acquiring vibrational Raman spectra, the protocol illustrates how to identify different chemical species within a mixture, determining their relative concentrations.

We present a protocol to acquire chemical images with broadband stimulated Raman scattering (SRS) microscopy. Based on an SRS microscope that operates with differential multichannel-lock-in detection, the protocol describes the sample preparation, adjustment of the SRS apparatus, and chemometrics to disentangle different constituents of chemically heterogeneous samples.

Geniş Bant Uyarılmış Raman Saçılma Mikroskobuna Dayalı Multipleks Kimyasal Görüntüleme

Stimulated Raman scattering (SRS) microscopy is a nonlinear optical technique for label-free chemical imaging. This analytical tool delivers chemical maps ...

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

Stimulated Raman scattering (SRS) and coherent anti-Stokes Raman scattering (CARS) microscopy are the most widely used coherent Raman scattering imaging technologies. Hyperspectral SRS and CARS imaging offer Raman spectral information at every pixel, which enables better separation of different chemical compositions. Although both techniques require two excitation lasers, their signal detection schemes and spectral properties are quite different. The goal of this protocol is to perform both hyperspectral SRS and CARS imaging on a single platform and compare the two microscopy techniques for imaging different biological samples. The spectral focusing method is employed to acquire spectral information using femtosecond lasers. By using standard chemical samples, the sensitivity, spatial resolution, and spectral resolution of SRS and CARS in the same excitation conditions (i.e., power at the sample, pixel dwell time, objective lens, pulse energy) are compared. The imaging contrasts of CARS and SRS for biological samples are juxtaposed and compared. The direct comparison of CARS and SRS performances would allow for optimal selection of the modality for chemical imaging.

This paper directly compares the resolution, sensitivity, and imaging contrasts of stimulated Raman scattering (SRS) and coherent anti-Stokes Raman scattering (CARS) integrated into the same microscope platform. The results show that CARS has a better spatial resolution, SRS gives better contrasts and spectral resolution, and both methods have similar sensitivity.

Kimyasal Görüntüleme için Hiperspektral Uyarılmış Raman Saçılması ve Tutarlı Anti-Stokes Raman Saçılma Mikroskobunun Doğrudan Karşılaştırılması

Stimulated Raman scattering (SRS) and coherent anti-Stokes Raman scattering (CARS) microscopy are the most widely used coherent Raman scattering imaging ...

Tek Nanoparçacıkların Heterojen Performansının Elektrokimyasal Yöntemlerle İncelenmesi

Tracking Electrochemistry on Single Nanoparticles with Surface-Enhanced Raman Scattering Spectroscopy and Microscopy

Studying electrochemical reactions on single nanoparticles is important to understand the heterogeneous performance of individual nanoparticles. This nanoscale heterogeneity remains hidden during the ensemble-averaged characterization of nanoparticles. Electrochemical techniques have been developed to measure currents from single nanoparticles but do not provide information about the structure and identity of the molecules that undergo reactions at the electrode surface. Optical techniques such as surface-enhanced Raman scattering (SERS) microscopy and spectroscopy can detect electrochemical events on individual nanoparticles while simultaneously providing information on the vibrational modes of electrode surface species. In this paper, a protocol to track the electrochemical oxidation-reduction of Nile Blue (NB) on single Ag nanoparticles using SERS microscopy and spectroscopy is demonstrated. First, a detailed protocol for fabricating Ag nanoparticles on a smooth and semi-transparent Ag film is described. A dipolar plasmon mode aligned along the optical axis is formed between a single Ag nanoparticle and Ag film. The SERS emission from NB fixed between the nanoparticle and the film is coupled into the plasmon mode, and the high-angle emission is collected by a microscope objective to form a donut-shaped emission pattern. These donut-shaped SERS emission patterns allow for the unambiguous identification of single nanoparticles on the substrate, from which the SERS spectra can be collected. In this work, a method for employing the SERS substrate as a working electrode in an electrochemical cell compatible with an inverted optical microscope is provided. Finally, tracking the electrochemical oxidation-reduction of NB molecules on an individual Ag nanoparticle is shown. The setup and the protocol described here can be modified to study various electrochemical reactions on individual nanoparticles.

Yazar Spotlight: Yüzeyde Geliştirilmiş Raman Saçılma Spektroskopisi ve Mikroskobu ile Tek Nanopartiküller Üzerinde Elektrokimyanın İzlenmesi  

The protocol describes how to monitor electrochemical events on single nanoparticles using surface-enhanced Raman scattering spectroscopy and imaging.

Yüzeyle Geliştirilmiş Raman Saçılma Spektroskopisi ve Mikroskobu ile Tek Nanopartiküller Üzerinde Elektrokimyanın İzlenmesi

Studying electrochemical reactions on single nanoparticles is important to understand the heterogeneous performance of individual nanoparticles. This ...

Lazer Ablasyon ile Nanopartiküllerin ve Nanoyapıların Sentezlenmesi

Ultrafast Laser-Ablated Nanoparticles and Nanostructures for Surface-Enhanced Raman Scattering-Based Sensing Applications

The technique of ultrafast laser ablation in liquids has evolved and matured over the past decade, with several impending applications in various fields such as sensing, catalysis, and medicine. The exceptional feature of this technique is the formation of nanoparticles (colloids) and nanostructures (solids) in a single experiment with ultrashort laser pulses. We have been working on this technique for the past few years, investigating its potential using the surface-enhanced Raman scattering (SERS) technique in hazardous materials sensing applications. Ultrafast laser-ablated substrates (solids and colloids) could detect several analyte molecules at the trace levels/mixture form, including dyes, explosives, pesticides, and biomolecules. Here, we present some of the results achieved using the targets of Ag, Au, Ag-Au, and Si. We have optimized the nanostructures (NSs) and nanoparticles (NPs) obtained (in liquids and air) using different pulse durations, wavelengths, energies, pulse shapes, and writing geometries. Thus, various NSs and NPs were tested for their efficiency in sensing numerous analyte molecules using a simple, portable Raman spectrometer. This methodology, once optimized, paves the way for on-field sensing applications. We discuss the protocols in (a) synthesizing the NPs/NSs via laser ablation, (b) characterization of NPs/NSs, and (c) their utilization in the SERS-based sensing studies.

Yazar Spotlight: Sıvılarda Lazer Ablasyon Yoluyla Nanopartikül Sentezindeki Gelişmeler ve Uygulamalar 

Ultrafast laser ablation in liquid is a precise and versatile technique for synthesizing nanomaterials (nanoparticles [NPs] and nanostructures [NSs]) in liquid/air environments. The laser-ablated nanomaterials can be functionalized with Raman-active molecules to enhance the Raman signal of analytes placed on or near the NSs/NPs.

Yüzeyi Geliştirilmiş Raman Saçılımı Tabanlı Algılama Uygulamaları için Ultra Hızlı Lazerle Kesilmiş Nanopartiküller ve Nanoyapılar

The technique of ultrafast laser ablation in liquids has evolved and matured over the past decade, with several impending applications in various fields ...

Kimyasal bulaşanların Hızlı Algılama için Raman Spektroskopisi Tahlili Geliştirilmiş A Filtre tabanlı Yüzey

Özet

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Geniş Bant Uyarılmış Raman Saçılma Mikroskobuna Dayalı Multipleks Kimyasal Görüntüleme

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