Results: Single Cell Resolution Protein Expression in Whole Mount Skin Preparations
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Conclusion
Transkript
The overall goal of this procedure is to visualize cell proliferation and protein expression patterns at the single cell level in three dimensions and to accurately assess the anatomy and pathology of the skin. This method can help answer key ques
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This report describes a CUBIC protocol to clarify full thickness mouse skin biopsies, and visualize protein expression patterns, proliferating cells, and sebocytes at the single cell resolution in 3D. This method enables accurate assessment of skin anatomy and pathology, and of abnormal epidermal phenotypes in genetically modified mouse lines.