Orientation of Zebrafish on Divot Arrays and Microstructured Channels
2:39
Microinjection of Zebrafish
4:20
Screening of Embryos Using the PDMS Imaging Device
5:45
Results: Microinjection and Imagingof Zebrafish Larvae Using Microstructured Devices
7:04
Conclusion
Transkript
The overall goal of this methodology is to make microinjection of zerbrafish larvae easier and more accurate. This method helps researchers using zebrafish model systems to immobilize zebrafish larvae in specific orientations in large arrays. The
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Microinjection of zebrafish embryos and larvae is a crucial but challenging technique used in many zebrafish models. Here, we present a range of microscale tools to aid in the stabilization and orientation of zebrafish for both microinjection and imaging.