Isolation of Monocyte-derived DC from Peripheral Blood
6:54
Preparation of Tumor-IgG Immune Complexes Followed by Activation of MoDC with Tumor-IgG
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Results: Isolation and Analysis of the Activation of MoDC with the Tumor Immune Complex
11:02
Conclusion
Transkript
This method is designed to help scientists to isolate monocyte-derived DCs from the blood and tumors of tumor-bearing mice in order to study their immunostimulatory properties. I think that the main advantage of this procedure compared to other me
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Monocyte-derived DC (MoDC) can sense minor amounts of danger-associated molecules and are therefore easily primed. We provide a detailed protocol for the isolation of MoDC from blood and tumors and their activation with immune complexes while highlighting key precautions that should be considered in order to avoid their premature activation.