Preparation of Mouse Embryonic Day 10.5 Pancreatic Progenitors
6:12
Culturing Dissociated Progenitors to Form Pancreatoids
6:50
Results: Generation of Pancreatoids that Retain Native Mesenchyme and Develop Endocrine Cells
8:37
Conclusion
Transkript
This method could help answer key questions in the pancreatic field by using these pancreatoids to understand how endocrine cells form in an architecturally and cellularly complex environment. The main advantage of this technique is that the pancr
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Here, we present a protocol to generate insulin expressing 3D murine pancreatoids from free-floating e10.5 dissociated pancreatic progenitors and the associated mesenchyme.