Sandwiching Cell Sheet Seeding and SWAT Supply Preparation
2:02
Gelatin Plunger Preparation
3:40
White Adipose Tissue Processing
5:49
Results: Representative SWAT Analyses
7:23
Conclusion
Transkript
This breakthrough method allows us, for the first time, to keep fat alive and healthy ex vivo for several consecutive weeks for the study of adipose biology. The main advantage of this technique is that the tissue remains stable over eight weeks i
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White adipose tissue (WAT) has critical deficiencies in its current primary culture models, hindering pharmacological development and metabolic studies. Here, we present a protocol to produce an adipose microphysiological system by sandwiching WAT between sheets of stromal cells. This construct provides a stable and adaptable platform for primary WAT culture.